Anti-RPN2 Antibody Picoband®, PE Conjugate

Boster Bio Anti-RPN2 Antibody Picoband® catalog # A05006-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit 2, also called ribophorin ∥ is an enzyme that in humans is encoded by the RPN2 gene. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Fms-related tyrosine kinase 3 ligand ;Flt3lg ;

Gene Name

fms-related tyrosine kinase 3 ligand

Gene ID

6185

UniProt

P04844

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RPN2 recombinant protein (Position: Q64-Q174).

Clonality

Polyclonal

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Barton, D. E., Crimaudo, C., Hortsch, M., Francke, U. The genes for ribophorins I and II are on human chromosomes 3q and 20 and mouse chromosomes 6 and 12, respectively. (Abstract) Cytogenet. Cell Genet. 46: 577 only, 1987. 2. Crimaudo, C., Hortsch, M., Gausepohl, H., Meyer, D. I. Human ribophorins I and II: the primary structure and membrane topology of two highly conserved round endoplasmic reticulum-specific glycoproteins. EMBO J. 6: 75-82, 1987. 3. Honma, K., Iwao-Koizumi, K., Takeshita, F., Yamamoto, Y., Yoshida, T., Nishio, K., Nagahara, S., Kato, K., Ochiya, T. RPN2 gene confers docetaxel resistance in breast cancer. Nature Med. 14: 939-948, 2008. Note: Erratum: Nature Med. 14: 1128 only, 2008.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

25847 MW

Observed Molecular Weight

69 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -