Anti-RIOK3 Antibody Picoband®, PE Conjugate

Boster Bio Anti-RIOK3 Antibody Picoband® catalog # A09262-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants.

Synonyms

Nck-associated protein 1;NAP 1;Membrane-associated protein HEM-2;p125Nap1;NCKAP1;HEM2, KIAA0587, NAP1;

Gene Name

Nck-associated protein 1

Gene ID

8780

UniProt

O14730

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RIOK3 recombinant protein (Position: Q48-E519).

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Anaya, P., Evans, S. C., Dai, C., Lozano, G., May, G. S. Isolation of the Aspergillus nidulans sudD gene and its human homologue. Gene 211: 323-329, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

128790 MW

Observed Molecular Weight

65 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Cell membrane ; Single-pass membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Single-pass membrane protein ; Cytoplasmic side . At the interface between the lamellipodial actin meshwork and the membrane. .