Anti-C18orf22/RBFA Antibody Picoband®, PE Conjugate

Boster Bio Anti-C18orf22/RBFA Antibody Picoband® catalog # A13257-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Predicted to be involved in rRNA processing. Predicted to be located in mitochondrion.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

79863

UniProt

Q8N0V3

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human C18orf22/RBFA recombinant protein (Position: L15-D319).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Kuwasako, K. , Suzuki, S. , Nameki, N. , Takizawa, M. , Takahashi, M. , & Tsuda, K. , et al. (2022). 1h, 13c, and 15n resonance assignments and solution structures of the kh domain of human ribosome binding factor a, mtrbfa, involved in mitochondrial ribosome biogenesis. Biomolecular NMR Assignments, 1-7. 2. Rozanska, A. , Richter-Dennerlein, R. , Rorbach, J. , Gao, F. , & Lightowlers, R. N. . (2017). The human rna-binding protein rbfa promotes the maturation of the mitochondrial ribosome. Biochemical Journal, 474(13), 2145-2158. 3. Hauri, S. , Wepf, A. , Drogen, A. V. , Varjosalo, M. , & Gstaiger, M. . (2013). Interaction proteome of human hippo signaling: modular control of the co-activator yap1. Molecular Systems Biology, 9(1), 713.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

40 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -