Anti-Sarcalumenin/Srl Antibody Picoband®, PE Conjugate

Boster Bio Anti-Sarcalumenin/Srl Antibody Picoband® catalog # A07699-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Sarcalumenin is a protein that in humans is encoded by the SRL gene. Predicted to enable GTP binding activity. Acts upstream of or within response to muscle activity involved in regulation of muscle adaptation and store-operated calcium entry. Predicted to be located in membrane and sarcoplasmic reticulum. Predicted to be active in cytoplasm; intracellular membrane-bounded organelle; and plasma membrane. Is expressed in brain ventricular layer; diaphragm; limb; skeletal musculature; and tail mesenchyme. Orthologous to human SRL (sarcalumenin).

Synonyms

Chromaffin granule amine transporter; Solute carrier family 18 member 1; Vesicular amine transporter 1; VAT1; SLC18A1; VAT1; VMAT1

Gene Name

solute carrier family 18 member A1

Gene ID

106393

UniProt

Q7TQ48

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Sarcalumenin/Srl recombinant protein (Position: R119-M422).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Leberer, E., Charuk, J. H. M., Clarke, D. M., Green, N. M., Zubrzycka-Gaarn, E., MacLennan, D. H. Molecular cloning and expression of cDNA encoding the 53,000-dalton glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum. J. Biol. Chem. 264: 3484-3493, 1989. 2. Leberer, E., Charuk, J. H. M., Green, N. M., MacLennan, D. H. Molecular cloning and expression of cDNA encoding a lumenal calcium binding glycoprotein from sarcoplasmic reticulum. Proc. Nat. Acad. Sci. 86: 6047-6051, 1989. 3. Scott, A. Personal Communication. Baltimore, Md. 9/30/1999.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

54 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse Flow Cytometry (Fixed), 1-3 μg/1x106 cellsMouse ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Cytoplasmic vesicle membrane. Multi-pass membrane protein. Synaptic vesicle membrane. Multi-pass membrane protein. Endoplasmic reticulum membrane. Multi-pass membrane protein.