Anti-SPACA3 Antibody Picoband®, PE Conjugate

Boster Bio Anti-SPACA3 Antibody Picoband® catalog # A10284-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Sperm acrosome membrane-associated protein 3 is a protein that in humans is encoded by the SPACA3 gene. The protein encoded by this gene is a sperm surface protein that may be involved in adhesion to the egg prior to fertilization. While the encoded protein has significant similarity to lysozyme at the amino acid level, it has no detectable bacteriocidal activity. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA

Gene Name

pre T cell antigen receptor alpha

Gene ID

124912

UniProt

Q8IXA5

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SPACA3 recombinant protein (Position: E86-F215).

Clonality

Polyclonal

Tissue Specificity

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Mandal, A., Klotz, K. L., Shetty, J., Jayes, F. L., Wolkowicz, M. J., Bolling, L. C., Coonrod, S. A., Black, M. B., Diekman, A. B., Haystead, T. A. J., Flickinger, C. J., Herr, J. C. SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. Biol. Reprod. 68: 1525-1537, 2003. 2. Zhang, K., Gao, R., Zhang, H., Cai, X., Shen, C., Wu, C., Zhao, S., Yu, L. Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the C-type lysozyme/alpha-lactalbumin family. Biol. Reprod. 73: 1064-1071, 2005.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

41409 MW

Observed Molecular Weight

40 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -