Anti-Thymidine Kinase 2/TK2 Antibody Picoband®, PE Conjugate

Boster Bio Anti-Thymidine Kinase 2/TK2 Antibody Picoband® catalog # A03321-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Thymidine kinase 2, mitochondrial is a protein that in humans is encoded by the TK2 gene. This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria.

Synonyms

Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5;

Gene Name

glutathione S-transferase mu 3

Gene ID

7084

UniProt

O00142

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Thymidine Kinase 2/TK2 recombinant protein (Position: V34-P265).

Clonality

Polyclonal

Tissue Specificity

Testis and brain.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Akman, H. O., Dorado, B., Lopez, L. C., Garcia-Cazorla, A., Vila, M. R., Tanabe, L. M., Dauer, W. T., Bonilla, E., Tanji, K., Hirano, M. Thymidine kinase 2 (H126N) knockin mice show the essential role of balanced deoxynucleotide pools for mitochondrial DNA maintenance. Hum. Molec. Genet. 17: 2433-2440, 2008. 2. Bartesaghi, S., Betts-Henderson, J., Cain, K., Dinsdale, D., Zhou, X., Karlsson, A., Salomoni, P., Nicotera, P. Loss of thymidine kinase 2 alters neuronal bioenergetics and leads to neurodegeneration. Hum. Molec. Genet. 19: 1669-1677, 2010. 3. Behin, A., Jardel, C., Claeys, K. G., Fagart, J., Louha, M., Romero, N. B., Laforet, P., Eymard, B., Lombes, A. Adult cases of mitochondrial DNA depletion due to TK2 defect: an expanding spectrum. Neurology 78: 644-648, 2012.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.