Anti-mSin3A/SIN3A Antibody Picoband®, PE Conjugate

Boster Bio Anti-mSin3A/SIN3A Antibody Picoband® catalog # A01203-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Paired amphipathic helix protein Sin3a is a protein that in humans is encoded by the SIN3A gene. The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Synonyms

Microtubule-associated protein 2; MAP-2; MAP2

Gene Name

microtubule-associated protein 2

Gene ID

25942

UniProt

Q96ST3

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human mSin3A/SIN3A recombinant protein (Position: Q8-E918).

Clonality

Polyclonal

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ayer, D. E., Lawrence, Q. A., Eisenman, R. N. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3. Cell 80: 767-776, 1995. 2. Balasubramanian, M., Dingemans, A. J. M., Albaba, S., Richardson, R., Yates, T. M., Cox, H., Douzgou, S., Armstrong, R., Sansbury, F. H., Burke, K. B., Fry, A. E., Ragge, N., and 27 others. Comprehensive study of 28 individuals with SIN3A-related disorder underscoring the associated mild cognitive and distinctive facial phenotype. Europ. J. Hum. Genet. 29: 625-636, 2021. 3. Duong, H. A., Robles, M. S., Knutti, D., Weitz, C. J. A molecular mechanism for circadian clock negative feedback. Science 332: 1436-1439, 2011.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

150 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -