Anti-SCYL2 Antibody Picoband®, PE Conjugate

Boster Bio Anti-SCYL2 Antibody Picoband® catalog # A08578-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SCY1-like protein 2 is a protein that in humans is encoded by the SCYL2 gene. The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene.

Synonyms

Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7;

Gene Name

Gap junction gamma-1 protein

Gene ID

55681

UniProt

Q6P3W7

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SCYL2 recombinant protein (Position: E208-Q909).

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Conner, S. D., Schmid, S. L. CVAK104 is a novel poly(L)-lysine-stimulated kinase that targets the beta-2-subunit of AP2. J. Biol. Chem. 280: 21539-21544, 2005. 2. Gingras, S., Earls, L. R., Howell, S., Smeyne, R. J., Zakharenko, S. S., Pelletier, S. SCYL2 protects CA3 pyramidal neurons from excitotoxicity during functional maturation of the mouse hippocampus. J. Neurosci. 35: 10510-10522, 2015. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/15/2015.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

45470 MW

Observed Molecular Weight

104 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -