Anti-SCML1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-SCML1 Antibody Picoband® catalog # A12084-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SCML1 Antibody (8-RY28) is a high quality monoclonal SCML1 antibody (also designated SCML1 antibody) suitable for the detection of the SCML1 protein of human origin. SCML1 Antibody (8-RY28) is available as the non-conjugated anti-SCML1 antibody. Putative Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. SCML1 (sex comb on midleg-like 1 (Drosophila)), is a 329 amino acid PcG protein belonging to the SCM family. Localizing to nucleus, SCML1 plays a role in testis development, spermatogenesis and embryonal development. While ubiquitously expressed, SCML1 is found at highest levels in adult skeletal muscle, heart and fetal liver. SCML1 contains one SAM (sterile alpha motif) domain and is encoded by a gene that spans 18 kb, contains 6 exons, maps to human chromosome Xp22.13, and is transcribed from telomere to centromere. Three SCML1 isoforms exist as a result of alternative splicing events.

Synonyms

Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182

Gene Name

negative elongation factor complex member B

Gene ID

6322

UniProt

Q9UN30

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SCML1 recombinant protein (Position: E8-K322).

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. van de Vosse, E., Walpole, S. M., Nicolaou, A., van der Bent, P., Cahn, A., Vaudin, M., Ross, M. T., Durham, J., Pavitt, R., Wilkinson, J., Grafham, D., Bergen, A. A. B., van Ommen, G.-J. B., Yates, J. R. W., den Dunnen, J. T., Trump, D. Characterization of SCML1, a new gene in Xp22, with homology to developmental polycomb genes. Genomics 49: 96-102, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

37 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -