Anti-NOB1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-NOB1 Antibody Picoband® catalog # A03504-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

RNA-binding protein NOB1 is a protein that in humans is encoded by the NOB1 gene. In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.

Synonyms

Rhombotin-2; Cysteine-rich protein TTG-2; LIM domain only protein 2; LMO-2; T-cell translocation protein 2; LMO2; RBTN2; RBTNL1; RHOM2; TTG2

Gene Name

LIM domain only 2

Gene ID

28987

UniProt

Q9ULX3

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NOB1 recombinant protein (Position: M1-D389).

Clonality

Polyclonal

Applications

WB,IHC,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ameismeier, M., Cheng, J., Berninghausen, O., Beckmann, R. Visualizing late states of human 40S ribosomal subunit maturation. Nature 558: 249-253, 2018. 2. Fatica, A., Oeffinger, M., Dlakic, M., Tollervey, D. Nob1p is required for cleavage of the 3-prime end of 18S rRNA. Molec. Cell. Biol. 23: 1798-1807, 2003. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/6/2010.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

47 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat<br> ELISA, 0.1-0.5 μg/ml, -<br>