Anti-TRMT6 Antibody Picoband®, PE Conjugate

Boster Bio Anti-TRMT6 Antibody Picoband® catalog # A13158. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants.

Synonyms

Follicular dendritic cell secreted peptide;FDC secreted protein;FDC-SP;FDCSP;C4orf7;UNQ733/PRO1419;

Gene Name

Follicular dendritic cell secreted peptide

Gene ID

51605

UniProt

Q9UJA5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human TRMT6 recombinant protein (Position: E6-K489).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Wang, Y. , Huang, Q. , Deng, T. , Li, B. H. , & Ren, X. Q. . (2019). Clinical significance of trmt6 in hepatocellular carcinoma: a bioinformatics-based study. Medical Science Monitor, 25, 3894-3901. 2. Finermoore, J. , Czudnochowski, N. , O'Connell, J. D. , Wang, A. L. , & Stroud, R. M. . (2015). Crystal structure of the human trna m1a58 methyltransferase–trna3lys complex: refolding of substrate trna allows access to the methylation target. Journal of Molecular Biology, 427(24), 3862-3876. 3. Wang, Y. , Wang, J. , Li, X. , Xiong, X. , Wang, J. , & Zhou, Z. , et al. N 1-methyladenosine methylation in trna drives liver tumourigenesis by regulating cholesterol metabolism. Nature communications, 12(1), 6314.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

9700 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -