Anti-CNPase/CNP Antibody Picoband®, PE Conjugate

Boster Bio Anti-CNPase/CNP Antibody Picoband® catalog # A01017-4. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles.

Synonyms

2',3'-cyclic-nucleotide 3'-phosphodiesterase;CNP;CNPase;3.1.4.37;CNP;

Gene Name

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Gene ID

1267

UniProt

P09543

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CNPase/CNP recombinant protein (Position: M1-I421).

Clonality

Polyclonal

Applications

WB,IHC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. "Entrez Gene: CNP 2',3'-cyclic nucleotide 3' phosphodiesterase". 2. Hinman JD, Chen CD, Oh SY, Hollander W, Abraham CR (Jan 2008). "Age-dependent accumulation of ubiquitinated 2',3'-cyclic nucleotide 3'-phosphodiesterase in myelin lipid rafts". Glia. 56 (1): 118–33. 3. Sprinkle TJ, Lanclos KD, Lapp DF (Jul 1992). "Assignment of the human 2',3'-cyclic nucleotide 3'-phosphohydrolase gene to chromosome 17".Genomics. 13 (3): 877–80.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

47579 MW

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Membrane; Lipid-anchor. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.