Anti-UBE2O Antibody Picoband®, PE Conjugate

Boster Bio Anti-UBE2O Antibody Picoband® catalog # A09217-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Ubiquitin-conjugating enzyme E2 O is a protein that in humans is encoded by the UBE2O gene. Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.

Synonyms

Homer protein homolog 3;Homer-3;HOMER3;

Gene Name

Homer protein homolog 3

Gene ID

63893

UniProt

Q9C0C9

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human UBE2O recombinant protein (Position: R150-A1283).

Clonality

Polyclonal

Tissue Specificity

Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Mashtalir, N., Daou, S., Barbour, H., Sen, N. N., Gagnon, J., Hammond-Martel, I., Dar, H. H., Therrien, M., Affar, E. B. Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Molec. Cell 54: 392-406, 2014. 2. Nagase, T., Kikuno, R., Hattori, A., Kondo, Y., Okumura, K., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 347-355, 2000. 3. Nguyen, A. T., Prado, M. A., Schmidt, P. J., Sendamarai, A. K., Wilson-Grady, J. T., Min, M., Campagna, D. R., Tian, G., Shi, Y., Dederer, V., Kawan, M., Kuehnle, N., Paulo, J. A., Yao, Y., Weiss, M. J., Justice, M. J., Gygi, S. P., Fleming, M. D., Finley, D. UBE2O remodels the proteome during terminal erythroid differentiation. Science 357: eaan0218, 2017. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39836 MW

Observed Molecular Weight

200 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Cytoplasm . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse . Postsynaptic density of neuronal cells. .