Anti-CAD Antibody Picoband®, PE Conjugate

Boster Bio Anti-CAD Antibody Picoband® catalog # A00463-4. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

CAD protein; Glutamine-dependent carbamoyl-phosphate synthase

Gene Name

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Gene ID

790

UniProt

P27708

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CAD recombinant protein (Position: R1403-D2198).

Clonality

Polyclonal

Applications

WB,IHC,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ben-Sahra, I., Howell, J. J., Asara, J. M., Manning, B. D. Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. Science 339: 1323-1328, 2013. 2. Bertoni, L., Attolini, C., Simi, S., Giulotto, E. Localization of the Chinese hamster CAD gene reveals homology between human chromosome 2p and Chinese hamster 7q. Genomics 16: 779-781, 1993. 3. Chen, K.-C., Vannais, D. B., Jones, C., Patterson, D., Davidson, J. N. Chromosomal localization of the human CAD gene to 2p21-22. (Abstract) Am. J. Hum. Genet. 41: A161 only, 1987.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

260 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>