Anti-KLC1 Antibody Picoband® (monoclonal, 4F2D7), PE Conjugate

Boster Bio Anti-KLC1 Antibody Picoband® (monoclonal, 4F2D7) catalog # M04116-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene. Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.

Synonyms

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2, UBLE1B; HRIHFB2115

Gene Name

ubiquitin like modifier activating enzyme 2

Gene ID

3831

UniProt

Q07866

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human KLC1 recombinant protein (Position: M1-D537).

Clonality

Monoclonal

Clone

Clone: 4F2D7

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Bracale, A., Cesca, F., Neubrand, V. E., Newsome, T. P., Way, M., Schiavo, G. Kidins220/ARMS is transported by a kinesin-1-based mechanism likely to be involved in neuronal differentiation. Molec. Biol. Cell 18: 142-152, 2007. 2. Cabeza-Arvelaiz, Y., Shih, L.-C. N., Hardman, N., Asselbergs, F., Bilbe, G., Schmitz, A., White, B., Siciliano, M. J., Lachman, L. B. Cloning and genetic organization of the human kinesin light-chain (KLC) gene. DNA Cell Biol. 12: 881-892, 1993. 3. Chernajovsky, Y., Brown, A., Clark, J. Human kinesin light (beta) chain gene: DNA sequence and functional characterization of its promoter and first exon. DNA Cell Biol. 15: 965-974, 1996.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.