Anti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1), PE Conjugate

Boster Bio Anti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) catalog # M04551. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Synonyms

Coatomer subunit epsilon; Epsilon-coat protein; Epsilon-COP; COPE

Gene Name

coatomer protein complex subunit epsilon

Gene ID

11052

UniProt

Q16630

Host

Mouse

Reactivity

Human,Mouse,Rat,Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CPSF6 recombinant protein (Position: R50-Q176).

Clonality

Monoclonal

Clone

Clone: 3F11E1

Tissue Specificity

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Rasaiyaah, J., Tan, C. P., Fletcher, A. J., Price, A. J., Blondeau, C., Hilditch, L., Jacques, D. A., Selwood, D. L., James, L. C., Noursadeghi, M., Towers, G. J. HIV-1 evades innate immune recognition through specific cofactor recruitment. Nature 503: 402-405, 2013. 2. Ruegsegger, U., Blank, D., Keller, W. Human pre-mRNA cleavage factor I-m is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Molec. Cell 1: 243-253, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

68 kDa

Fragment

Mouse IgG1

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human