Anti-MSF/SEPTIN9 Antibody Picoband®, PE Conjugate

Boster Bio Anti-MSF/SEPTIN9 Antibody Picoband® catalog # A05279-3. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Synonyms

Aldo-keto reductase family 1 member D1; 3-oxo-5-beta-steroid 4-dehydrogenase; Delta (4)-3-ketosteroid 5-beta-reductase; Delta (4)-3-oxosteroid 5-beta-reductase; AKR1D1; SRD5B1

Gene Name

aldo-keto reductase family 1 member D1

Gene ID

10801

UniProt

Q9UHD8

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MSF/SEPTIN9 recombinant protein (Position: R15-D282).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in liver. Expressed in testis and weakly in colon.

Applications

ELISA,IF,IHC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta4-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-4-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Collie, A. M. B., Landsverk, M. L., Ruzzo, E., Mefford, H. C., Buysse, K., Adkins, J. R., Knutzen, D. M., Barnett, K., Brown, R. H., Jr., Parry, G. J., Yum, S. W., Simpson, D. A., Olney, R. K., Chinnery, P. F., Eichler, E. E., Chance, P. F., Hannibal, M. C. Non-recurrent SEPT9 duplications cause hereditary neuralgic amyotrophy. J. Med. Genet. 47: 601-607, 2010. 2. Hannibal, M. C., Ruzzo, E. K., Miller, L. R., Betz, B., Buchan, J. G., Knutzen, D. M., Barnett, K., Landsverk, M. L., Brice, A., LeGuern, E., Bedford, H. M., Worrall, B. B., Lovitt, S., Appel, S. H., Andermann, E., Bird, T. D., Chance, P. F. SEPT9 gene sequencing analysis reveals recurrent mutations in hereditary neuralgic amyotrophy. Neurology 72: 1755-1759, 2009. 3. Kalikin, L. M., Sims, H. L., Petty, E. M. Genomic and expression analyses of alternatively spliced transcripts of the MLL septin-like fusion gene (MSF) that map to a 17q25 region of loss in breast and ovarian tumors. Genomics 63: 165-172, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

70 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -