Anti-NOP58 Antibody Picoband®, PE Conjugate

Boster Bio Anti-NOP58 Antibody Picoband® catalog # A05070-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nucleolar protein 58 is a protein that in humans is encoded by the NOP58 gene. The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

Synonyms

Matrix metalloproteinase-16; MMP-16; 3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP; MMP16; MMPX2;

Gene Name

matrix metallopeptidase 16

Gene ID

51602

UniProt

Q9Y2X3

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NOP58 recombinant protein (Position: K30-K450).

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/8/2016. 2. Lyman, S. K., Gerace, L., Baserga, S. J. Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins. RNA 5: 1597-1604, 1999. 3. Nelson, S. A., Santora, K. E., LaRochelle, W. J. Isolation and characterization of a novel PDGF-induced human gene. Gene 253: 87-93, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39125 MW

Observed Molecular Weight

70 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>