Anti-RAD18 Antibody Picoband®, PE Conjugate

Boster Bio Anti-RAD18 Antibody Picoband® catalog # A01622-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Synonyms

Guanine deaminase; Guanase; Guanine aminase; Guanine aminohydrolase; GAH; p51-nedasin; GDA; KIAA1258

Gene Name

guanine deaminase

Gene ID

56852

UniProt

Q9NS91

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RAD18 recombinant protein (Position: M1-D408).

Clonality

Polyclonal

Tissue Specificity

Expressed in lung carcinoma cell lines but not in normal lung tissues.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Adams, D. J., van der Weyden, L., Gergely, F. V., Arends, M. J., Ng, B. L., Tannahill, D., Kanaar, R., Markus, A., Morris, B. J., Bradley, A. BRCTx is a novel, highly conserved RAD18-interacting protein. Molec. Cell. Biol. 25: 779-788, 2005. 2. Branzei, D., Vanoli, F., Foiani, M. SUMOylation regulates Rad18-mediated template switch. Nature 456: 915-920, 2008. 3. Centore, R. C., Yazinski, S. A., Tse, A., Zou, L. Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response. Molec. Cell 46: 625-635, 2012.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>