Anti-CSDE1/NRU Antibody Picoband®, PE Conjugate

Boster Bio Anti-CSDE1/NRU Antibody Picoband® catalog # A05114-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Cold shock domain-containing protein E1 is a protein that in humans is encoded by the CSDE1 gene. Originally described as the product of an active transcription unit located upstream of the N-RAS gene, CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains. CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner. CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs. In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis.

Synonyms

Sodium- and chloride-dependent GABA transporter 1;GAT-1;Solute carrier family 6 member 1;SLC6A1;GABATR, GABT1, GAT1;

Gene Name

Sodium- and chloride-dependent GABA transporter 1

Gene ID

7812

UniProt

O75534

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CSDE1/NRU recombinant protein (Position: M1-Q775).

Clonality

Polyclonal

Tissue Specificity

Expressed in many tissues, highest levels in skeletal muscle.

Applications

ELISA,Flow Cytometry,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Chang, T.-C., Yamashita, A., Chen, C.-Y. A., Yamashita, Y., Zhu, W., Durdan, S., Kahvejian, A., Sonenberg, N., Shyu, A.-B. UNR, a new partner of poly(A)-binding protein, plays a key role in translationally coupled mRNA turnover mediated by the c-fos major coding-region determinant. Genes Dev. 18: 2010-2023, 2004. 2. Doniger, J., DiPaolo, J. A. Coordinate N-RAS mRNA up-regulation with mutational activation in tumorigenic guinea pig cells. Nucleic Acids Res. 16: 969-980, 1988. 3. Edwards, M. C., Liegeois, N., Horecka, J., DePinho, R. A., Sprague, G. F., Jr., Tyers, M., Elledge, S. J. Human CPR (cell cycle progression restoration) genes impart a Far- phenotype on yeast cells. Genetics 147: 1063-1076, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

67074 MW

Observed Molecular Weight

89 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). .