Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7), PE Conjugate

Boster Bio Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7) catalog # M06559. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Semaphorin-3Bis aproteinthat in humans is encoded by theSEMA3Bgene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.

Synonyms

T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5

Gene Name

chaperonin containing TCP1 subunit 3

Gene ID

7869

UniProt

Q13214

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).

Clonality

Monoclonal

Clone

Clone: 9C4F7

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

WB,ICC,IF,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Ochi K, Mori T, Toyama Y, Nakamura Y, Arakawa H (2002). "Identification of semaphorin3B as a target of p53". Neoplasia. 4 (1): 82–7. 2. Tse C, Xiang RH, Bracht T, Naylor SL (January 2002). "Human Semaphorin 3B (SEMA3B) located at chromosome 3p21.3 suppresses tumor formation in an adenocarcinoma cell line". Cancer Research. 62 (2): 542–6.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

83 kDa

Fragment

Mouse IgG2b

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human