Anti-CENPH Antibody Picoband®, PE Conjugate

Boster Bio Anti-CENPH Antibody Picoband® catalog # A06302-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Centromere protein H is a protein that in humans is encoded by the CENPH gene. Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Synonyms

ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL2; HUB

Gene Name

ELAV like RNA binding protein 2

Gene ID

64946

UniProt

Q9H3R5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CENPH recombinant protein (Position: E37-M247).

Clonality

Polyclonal

Tissue Specificity

Brain; neural-specific.

Applications

ELISA,Flow Cytometry,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Earnshaw, W. C. When is a centromere not a kinetochore? J. Cell Sci. 99: 1-4, 1991. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/4/2014. 3. Sugata, N., Li, S., Earnshaw, W. C., Yen, T. J., Yoda, K., Masumoto, H., Munekata, E., Warburton, P. E., Todokoro, K. Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes. Hum. Molec. Genet. 9: 2919-2926, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.