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Anti-IFIT5 Antibody Picoband®, PE Conjugate

Boster Bio Anti-IFIT5 Antibody Picoband® catalog # A07415-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Synonyms

BAG family molecular chaperone regulator 5;BAG-5;Bcl-2-associated athanogene 5;BAG5;KIAA0873;

Gene Name

BAG family molecular chaperone regulator 5

Gene ID

24138

UniProt

Q13325

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human IFIT5 recombinant protein (Position: N23-S439).

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues examined with lower levels in brain and testis.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Abbas, Y. M., Pichlmair, A., Gorna, M. W., Superti-Furga, G., Nagar, B. Structural basis for viral 5-prime-PPP-RNA recognition by human IFIT proteins. Nature 494: 60-64, 2013. 2. Feng, F., Yuan, L., Wang, Y. E., Crowley, C., Lv, Z., Li, J., Liu, Y., Cheng, G., Zeng, S., Liang, H. Crystal structure and nucleotide selectivity of human IFIT5/ISG58. (Letter) Cell Res. 23: 1055-1058, 2013. 3. Gross, M. B. Personal Communication. Baltimore, Md. 12/18/2014.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

51200 MW

Observed Molecular Weight

56 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Perinuclear region.

Protein Name

BAG family molecular chaperone regulator 5

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