Anti-EDC4 Antibody Picoband®, PE Conjugate

Boster Bio Anti-EDC4 Antibody Picoband® catalog # A05173-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Enhancer of mRNA-decapping protein 4 is a protein that in humans is encoded by the EDC4 gene. Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjgren's syndrome patient later diagnosed with primary biliary cirrhosis. EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation. Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182. Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression.

Synonyms

Protein Dok-7; Downstream of tyrosine kinase 7; DOK7; C4orf25;

Gene Name

docking protein 7

Gene ID

23644

UniProt

Q6P2E9

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EDC4 recombinant protein (Position: K1082-L1388).

Clonality

Polyclonal

Tissue Specificity

Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen .

Applications

ELISA,IF,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bloch, D. B., Rabkina, D., Quertermous, T., Bloch, K. D. The immunoreactive region in a novel autoantigen contains a nuclear localization sequence. Clin. Immun. Immunopath. 72: 380-389, 1994. 2. Fenger-Gron, M., Fillman, C., Norrild, B., Lykke-Andersen, J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Molec. Cell 20: 905-915, 2005. 3. Garcia-Lozano, J. R., Gonzalez-Escribano, M. F., Wichmann, I., Nunez-Roldan, A. Cytoplasmic detection of a novel protein containing a nuclear localization sequence by human autoantibodies. Clin. Exp. Immun. 107: 501-506, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

67074 MW

Observed Molecular Weight

152 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -