Anti-KCNQ2 Antibody Picoband®, PE Conjugate

Boster Bio Anti-KCNQ2 Antibody Picoband® catalog # A01259-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

KCNQ2, also called kv7.2, is a potassium channel protein coded for by the gene KCNQ2. It is mapped to 20q13.33. The KCNQ2 gene encodes a voltage-gated potassium channel that is expressed in the brain. Expression of human KCNQ2 in Xenopus laevis oocytes led to potassium-selective currents that activated slowly with depolarization. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1 (EBN1). At least five transcript variants encoding five different isoforms have been found for this gene.

Synonyms

Four and a half LIM domains protein 1; FHL-1; KyoT; RBP-associated molecule 14-1; RAM14-1; Skeletal muscle LIM-protein 1; SLIM; SLIM-1

Gene Name

four and a half LIM domains 1

Gene ID

3785

UniProt

O43526

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human KCNQ2 recombinant protein (Position: M371-D408).

Clonality

Polyclonal

Tissue Specificity

Isoform 1 seems to be most abundant in each tissue and isoform 2 much less abundant. Isoform 1 is highly expressed in skeletal muscle and lung, and to a lesser extent in heart, brain and kidney. Isoform 2 was found in brain, lung kidney and genital organs.

Applications

ELISA,Flow Cytometry,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

References & Citations

1. Biervert, C., Schroeder, B. C., Kubisch, C., Berkovic, S. F., Propping, P., Jentsch, T. J., Steinlein, O. K. A potassium channel mutation in neonatal human epilepsy. Science 279: 403-406, 1998. 2. "Entrez Gene: KCNQ2 potassium voltage-gated channel, KQT-like subfamily, member 2" 3. Heron, S. E., Cox, K., Grinton, B. E., Zuberi, S. M., Kivity, S., Afawi, Z., Straussberg, R., Berkovic, S. F., Scheffer, I. E., Mulley, J. C. Deletions or duplications in KCNQ2 can cause benign familial neonatal seizures. (Letter) J. Med. Genet. 44: 791-796, 2007.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

97 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Rat ELISA, 0.1-0.5μg/ml, -