Anti-Caspase-5/CASP5 Antibody Picoband®, PE Conjugate

Boster Bio Anti-Caspase-5/CASP5 Antibody Picoband® catalog # A05259-4. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.

Synonyms

Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1 factor; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA

Gene Name

MAF bZIP transcription factor A

Gene ID

838

UniProt

P51878

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Caspase-5/CASP5 recombinant protein (Position: D137-N434).

Clonality

Polyclonal

Tissue Specificity

Preferentially expressed in regulatory T-cells (Tregs).

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that activates insulin gene expression. Acts synergistically with NEUROD1/BETA2 and PDX1. Binds the insulin enhancer C1/RIPE3b element. Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Munday, N. A., Vaillancourt, J. P., Ali, A., Casano, F. J., Miller, D. K., Molineaux, S. M., Yamin, T.-T., Yu, V. L., Nicholson, D. W. Molecular cloning and pro-apoptotic activity of ICE(rel)II and ICE(rel)III, members of the ICE/CED-3 family of cysteine proteases. J. Biol. Chem. 270: 15870-15876, 1995. 2. Nasir, J., Theilmann, J. L., Vaillancourt, J. P., Munday, N. A., Ali, A., Scherer, S., Beatty, B., Nicholson, D. W., Hayden, M. R. Interleukin-1-beta-converting enzyme (ICE) and related cell death genes ICE(rel)-II and ICE(rel)-III map to the same PAC clone at band 11q22.2-22.3. Mammalian Genome 8: 611-613, 1997.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -