Anti-MitoNEET/CISD1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-MitoNEET/CISD1 Antibody Picoband® catalog # A04360-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity. This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.

Synonyms

Urokinase-type plasminogen activator; U-plasminogen activator; uPA; Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B; PLAU

Gene Name

plasminogen activator, urokinase

Gene ID

55847

UniProt

Q9NZ45

Host

Rabbit

Reactivity

Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MitoNEET/CISD1 recombinant protein (Position: Y28-E93).

Clonality

Polyclonal

Tissue Specificity

Expressed in the prostate gland and prostate cancers.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Colca, J. R., McDonald, W. G., Waldon, D. J., Leone, J. W., Lull, J. M., Bannow, C. A., Lund, E. T., Mathews, W. R. Identification of a novel mitochondrial protein ('mitoNEET') cross-linked specifically by a thiazolidinedione photoprobe. Am. J. Physiol. Endocr. Metab. 286: E252-60, 2004. 2. Hou, X., Liu, R., Ross, S., Smart, E. J., Zhu, H., Gong, W. Crystallographic studies of human MitoNEET. J. Biol. Chem. 282: 33242-33246, 2007. 3. Lin, J., Zhou, T., Ye, K., Wang, J. Crystal structure of human mitoNEET reveals distinct groups of iron-sulfur proteins. Proc. Nat. Acad. Sci. 104: 14640-14645, 2007.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

15 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -

Subcellular Location

Secreted.

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