Anti-GAP43 Antibody Picoband®, PE Conjugate

Boster Bio Anti-GAP43 Antibody Picoband® catalog # A01868-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Growth Associated Protein 43 (GAP43) is a protein encoded by the GAP43 gene in humans. It is mapped to 3q13.31. The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Synonyms

Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46; GAP43

Gene Name

growth associated protein 43

Gene ID

14432

UniProt

P06837

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse GAP43 recombinant protein (Position: M1-A227).

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.

Applications

ELISA,Flow Cytometry,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Kosik, K. S., Orecchio, L. D., Bruns, G. A. P., Benowitz, L. I., MacDonald, G. P., Cox, D. R., Neve, R. L. Human GAP-43: its deduced amino acid sequence and chromosomal localization in mouse and human. Neuron 1: 127-132, 1988. 2. Reeves, R. H., O'Hara, B. F., Gearhart, J. D. Localization on mouse chromosome 16 of Smst and Gap43: a conserved synteny with human chromosome 3. (Abstract) Cytogenet. Cell Genet. 51: 1064, 1989. 3. Strittmatter, S. M., Fankhauser, C., Huang, P. L., Mashimo, H., Fishman, M. C. Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43. Cell 80: 445-452, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50433 MW

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -

Subcellular Location

Cell membrane. Peripheral membrane protein. Cytoplasmic side. Growth cone membrane. Filopodium membrane. Synapse. Perikaryon. Dendrite. Axon. Cytoplasm.