Anti-EYA4 Antibody Picoband®, PE Conjugate

Boster Bio Anti-EYA4 Antibody Picoband® catalog # A04516-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms

Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF; VEGFB; VRF

Gene Name

vascular endothelial growth factor B

Gene ID

2070

UniProt

O95677

Host

Rabbit

Reactivity

Human,Monkey,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EYA4 recombinant protein (Position: R28-E205).

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Borsani, G., DeGrandi, A., Ballabio, A., Bulfone, A., Bernard, L., Banfi, S., Gattuso, C., Mariani, M., Dixon, M., Donnai, D., Metcalfe, K., Winter, R., Robertson, M., Axton, R., Brown, A., van Heyningen, V., Hanson, I. EYA4, a novel vertebrate gene related to Drosophila eyes absent. Hum. Molec. Genet. 8: 11-23, 1999. 2. Depreux, F. F. S., Darrow, K., Conner, D. A., Eavey, R. D., Liberman, M. C., Seidman, C. E., Seidman, J. G. Eya4-deficient mice are a model for heritable otitis media. J. Clin. Invest. 118: 651-658, 2008. 3. Hildebrand, M. S., Coman, D., Yang, T., Gardner, R. J. M., Rose, E., Smith, R. J. H., Bahlo, M., Dahl, H.-H. M. A novel splice site mutation in EYA4 causes DFNA10 hearing loss. Am. J. Med. Genet. 143A: 1599-1604, 2007. Note: Erratum: Am. J. Med. Genet. 146A: 1099 only, 2008.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Monkey, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -