Anti-Musashi 1/Msi1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-Musashi 1/Msi1 Antibody Picoband® catalog # A05052-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.

Synonyms

RNA-binding protein Musashi homolog 1; Musashi-1; MSI1

Gene Name

musashi RNA binding protein 1

Gene ID

4440

UniProt

O43347

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Musashi 1/Msi1 recombinant protein (Position: Q33-S347).

Clonality

Polyclonal

Tissue Specificity

Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Applications

ELISA,Flow Cytometry,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Chavali, P. L., Stojic, L., Meredith, L. W., Joseph, N., Nahorski, M. S., Sanford, T. J., Sweeney, T. R., Krishna, B. A., Hosmillo, M., Firth, A. E., Bayliss, R., Marcelis, C. L., Lindsay, S., Goodfellow, I., Woods, C. G., Gergely, F. Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication. Science 357: 83-88, 2017. 2. Good, P., Yoda, A., Sakakibara, S., Yamamoto, A., Imai, T., Sawa, H., Ikeuchi, T., Tsuji, S., Satoh, H., Okano, H. The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells. Genomics 52: 382-384, 1998. 3. Siddall, N. A., McLaughlin, E. A., Marriner, N. L., Hime, G. R. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc. Nat. Acad. Sci. 103: 8402-8407, 2006.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.