Anti-GPNMB/Gpnmb Picoband® Antibody, PE Conjugate

Boster Bio Anti-GPNMB/Gpnmb Picoband® Antibody catalog # A02439-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Transmembrane glycoprotein NMB; DC-HIL; Dendritic cell-associated transmembrane protein; Osteoactivin; Gpnmb; Dchil; Hgfin;;Nmb

Gene Name

glycoprotein (transmembrane) nmb

Gene ID

93695

UniProt

Q99P91

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).

Clonality

Polyclonal

Tissue Specificity

Monocyte/macrophage specific.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Could be a melanogenic enzyme.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Lu, H., Wang, X., Pullen, M., Guan, H., Chen, H., Sahu, S., Zhang, B., Chen, H., Williams, R. W., Geisert, E. E., Lu, L., Jablonski, M. M. Genetic dissection of the Gpnmb network in the eye. Invest. Ophthal. Vis. Sci. 52: 4132-4142, 2011. 2. Weterman, M. A. J., Ajubi, N., van Dinter, I. M. R., Degen, W. G. J., van Muijen, G. N. P., Ruiter, D. J., Bloemers, H. P. J. Nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int. J. Cancer 60: 73-81, 1995. 3. Yang, C.-F., Lin, S.-P., Chiang, C.-P., Wu, Y.-H., H'ng, W. S., Chang, C.-P., Chen, Y.-T., Wu, J.-Y. Loss of GPNMB causes autosomal-recessive amyloidosis cutis dyschromica in humans. Am. J. Hum. Genet. 102: 219-232, 2018.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38779 MW

Observed Molecular Weight

64 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse ELISA, 0.1-0.5μg/ml, -