Anti-Carbonic Anhydrase 4/CA4 Picoband® Antibody, PE Conjugate

Boster Bio Anti-Carbonic Anhydrase 4/CA4 Picoband® Antibody catalog # A01523-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. It is mapped to 11; 11 C. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport.

Synonyms

Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; Ca4; Car4

Gene Name

carbonic anhydrase 4

Gene ID

12351

UniProt

Q64444

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Carbonic Anhydrase 4/CA4 recombinant protein (Position: E25-L291).

Clonality

Polyclonal

Tissue Specificity

Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels. .

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Alvarez, B. V., Vithana, E. N., Yang, Z., Koh, A. H., Yeung, K., Yong, V., Shandro, H. J., Chen, Y., Kolatkar, P., Palasingam, P., Zhang, K., Aung, T., Casey, J. R. Identification and characterization of a novel mutation in the carbonic anhydrase IV gene that causes retinitis pigmentosa. Invest. Ophthal. Vis. Sci. 48: 3459-3468, 2007. 2. Bardien, S., Ebenezer, N., Greenberg, J., Inglehearn, C. F., Bartmann, L., Goliath, R., Beighton, P., Ramesar, R., Bhattacharya, S. S. An eighth locus for autosomal dominant retinitis pigmentosa is linked to chromosome 17q. Hum. Molec. Genet. 4: 1459-1462, 1995. 3. Bardien, S., Ramesar, R., Bhattacharya, S., Greenberg, J. Retinitis pigmentosa locus on 17q (RP17): fine localization to 17q22 and exclusion of the PDEG and TIMP2 genes. Hum. Genet. 101: 13-17, 1997.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35032 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse ELISA, 0.1-0.5μg/ml, -