Anti-Scramblase 1/PLSCR1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-Scramblase 1/PLSCR1 Antibody Picoband® catalog # A02995-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Phospholipid scramblase 1 is a protein that in human is encoded by the PLSCR1 gene. This gene is mapped to 3q24.

Synonyms

Phospholipid scramblase 1; PL scramblase 1; Ca (2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b; PLSCR1

Gene Name

phospholipid scramblase 1

Gene ID

5359

UniProt

O15162

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Scramblase 1/PLSCR1 recombinant protein (Position: M1-S54).

Clonality

Polyclonal

Tissue Specificity

Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May mediate accelerated ATP-independent biional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Basse, F., Stout, J. G., Sims, P. J., Wiedmer, T. Isolation of an erythrocyte membrane protein that mediates Ca(2+)-dependent transbilayer movement of phospholipid. J. Biol. Chem. 271: 17205-17210, 1996. 2. Kametaka, S., Shibata, M., Moroe, K., Kanamori, S., Ohsawa, Y., Waguri, S., Sims, P. J., Emoto, K., Umeda, M., Uchiyama, Y. Identification of phospholipid scramblase 1 as a novel interacting molecule with beta-secretase (beta-site amyloid precursor protein (APP) cleaving enzyme (BACE). J. Biol. Chem. 278: 15239-15245, 2003. 3. Kasukabe, T., Kobayashi, H., Kaneko, Y., Okabe-Kado, J., Honma, Y. Identity of human normal counterpart (MmTRA1b) of mouse leukemogenesis-associated gene (MmTRA1a) product as a plasma membrane phospholipid scramblase and chromosome mapping of the human MmTRA1b/phospholipid scramblase gene. Biochem. Biophys. Res. Commun. 249: 449-455, 1998.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36020 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Nucleus. Plasma membrane. Cell membrane. Single-pass type II membrane protein. Membrane. Lipid-anchor. Cytoplasmic side. Cytoplasm. Perinuclear region.