Anti-NRAMP1/SLC11A1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-NRAMP1/SLC11A1 Antibody Picoband® catalog # A02547-3. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Natural resistance-associated macrophage protein 1 is a protein that in humans is encoded by the SLC11A1 gene. It is mapped to 2q35. This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.

Synonyms

Natural resistance-associated macrophage protein 1; NRAMP 1; Solute carrier family 11 member 1; SLC11A1; LSH; NRAMP; NRAMP1

Gene Name

solute carrier family 11 member 1

Gene ID

6556

UniProt

P49279

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NRAMP1/SLC11A1 recombinant protein (Position: F183-S549).

Clonality

Polyclonal

Tissue Specificity

Macrophages; peripheral blood leukocytes, lung, spleen and liver.

Applications

ELISA,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Dubaniewicz, A., Jamieson, S. E., Dubaniewicz-Wybieralska, M., Fakiola, M., Miller, E. N., Blackwell, J. M. Association between SLC11A1 (formerly NRAMP1) and the risk of sarcoidosis in Poland. Europ. J. Hum. Genet. 13: 829-834, 2005. 2. Mohamed, H. S., Ibrahim, M. E., Miller, E. N., White, J. K., Cordell, H. J., Howson, J. M. M., Peacock, C. S., Khalil, E. A. G., El Hassan, A. M., Blackwell, J. M. SLC11A1 (formerly NRAMP1) and susceptibility to visceral leishmaniasis in The Sudan. Europ. J. Hum. Genet. 12: 66-74, 2004.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

90-120 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunocytochemistry/Immunofluorescence, 2μg/ml<br> ELISA, 0.1-0.5μg/ml<br>