Anti-Semaphorin 3B/SEMA3B Antibody Picoband®, PE Conjugate

Boster Bio Anti-Semaphorin 3B/SEMA3B Antibody Picoband® catalog # A06559-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Semaphorin-3B is a protein that in humans is encoded by the SEMA3B gene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.

Synonyms

Semaphorin-3B; Sema A (V); Semaphorin-V; Sema V; SEMA3B; SEMA5; SEMAA

Gene Name

semaphorin 3B

Gene ID

7869

UniProt

Q13214

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).

Clonality

Polyclonal

Tissue Specificity

Expressed abundantly but differentially in a variety of neural and nonneural tissues.

Applications

ELISA,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Ochi K, Mori T, Toyama Y, Nakamura Y, Arakawa H (2002). "Identification of semaphorin3B as a target of p53". Neoplasia. 4 (1): 82–7. 2. Tse C, Xiang RH, Bracht T, Naylor SL (January 2002). "Human Semaphorin 3B (SEMA3B) located at chromosome 3p21.3 suppresses tumor formation in an adenocarcinoma cell line". Cancer Research. 62 (2): 542–6.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

104553 MW

Observed Molecular Weight

83 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunocytochemistry/Immunofluorescence, 2μg/ml<br> ELISA, 0.1-0.5μg/ml<br>