Anti-SGLT2/SLC5A2 Antibody Picoband®, PE Conjugate

Boster Bio Anti-SGLT2/SLC5A2 Antibody Picoband® catalog # A03748-1. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The sodium/glucose cotransporter 2 (SGLT2) is a protein that in humans is encoded by the SLC5A2 gene. It is mapped to 16p11.2. This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene.

Synonyms

Sodium/glucose cotransporter 2; Na (+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2; SLC5A2; SGLT2

Gene Name

solute carrier family 5 member 2

Gene ID

6524

UniProt

P31639

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SGLT2/SLC5A2 recombinant protein (Position: A15-N656).

Clonality

Polyclonal

Applications

ELISA,Flow Cytometry,IHC,IHC-F,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Sodium-dependent glucose transporter. Has a Na+ to glucose coupling ratio of 1:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na+/glucose cotransporter arranged in series along kidney proximal tubules.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Calado, J., Soto, K., Clemente, C., Correia, P., Rueff, J. Novel compound heterozygous mutations in SLC5A2 are responsible for autosomal recessive renal glucosuria. Hum. Genet. 114: 314-316, 2004. 2. Wells, R. G., Pajor, A. M., Kanai, Y., Turk, E., Wright, E. M., Hediger, M. A. Cloning of a human kidney cDNA with similarity to the sodium-glucose cotransporter. Am. J. Physiol. 263: F459-F465, 1992.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

73 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells ELISA, 0.1-0.5μg/ml