Anti-Dopamine Receptor D1/DRD1 Antibody Picoband®, Dylight594 Conjugate

Boster Bio Anti-Dopamine Receptor D1/DRD1 Antibody catalog # PA1571. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Dopamine receptor D1, also known as DRD1, is a human gene. It is the most highly expressed DA receptor subtype among the DA receptor family. Receptors for dopamine have been classified into two functional types, D1 and D2. They belong to the family of receptors acting through G (or guanine nucleotide-binding) proteins. D2 receptors inhibit adenylyl cyclase, but D1 receptors stimulate adenylyl cyclase and activate cyclic AMP-dependent protein kinases. Dopamine D1 and D2 receptors are targets of drug therapy in many psychomotor disorders, including Parkinson's disease and schizophrenia, and may also have a role in drug addiction and alcoholism. D1 receptors regulate neuron growth and differentiation, influence behaviour and modify dopamine D2 receptor-mediated events. And the presence of a D1 receptor gene restriction fragment length polymorphism will be helpful for future disease linkage studies. DRD1 also regulates the neurochemical architecture of the striatum and is critical for the normal expression of motor activity.

Synonyms

D (1A) dopamine receptor;Dopamine D1 receptor;DRD1;

Gene Name

D(1A) dopamine receptor

UniProt

P21728

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human DRD1, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Detected in caudate, nucleus accumbens and in the olfactory tubercle. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Zhang J, Xiong B, Zhen X, Zhang A. (2009). "Dopamine D1 receptor ligands: where are we now and where are we going.". Med Res Rev. 29 (2): 272-294. 2. Sunahara, R. K.; Niznik, H. B.; Weiner, D. M.; Stormann, T. M.; Brann, M. R.; Kennedy, J. L.; Gelernter, J. E.; Rozmahel, R.; Yang, Y.; Israel, Y.; Seeman, P.; O'Dowd, B. F. :Human dopamine D1 receptor encoded by an intronless gene on chromosome 5. Nature 347: 80-83, 1990. 3. Xu, M.; Moratalla, R.; Gold, L. H.; Hiroi, N.; Koob, G. F.; Graybiel, A. M.; Tonegawa, S. : Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses. Cell 79: 729-742, 1994.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49293 MW

Observed Molecular Weight

130 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat <br>Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse<br>

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. .