Anti-L3MBTL3 Antibody Picoband®, Dylight594 Conjugate

Boster Bio Anti-L3MBTL3 Antibody Picoband® catalog # A10834-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.

Synonyms

RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3

Gene Name

NOVA alternative splicing regulator 2

Gene ID

84456

UniProt

Q96JM7

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human L3MBTL3 recombinant protein (Position: Q11-R686).

Clonality

Polyclonal

Tissue Specificity

Brain. Expression restricted to astrocytes.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May regulate RNA splicing or metabolism in a specific subset of developing neurons. Binds single strand RNA.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Arai, S., Miyazaki, T. Impaired maturation of myeloid progenitors in mice lacking novel Polycomb group protein MBT-1. EMBO J. 24: 1863-1873, 2005. 2. Xu, T., Park, S.-S., Giaimo, B. D., Hall, D., Ferrante, F., Ho, D. M., Hori, K., Anhezini, L., Ertl, I., Bartkuhn, M., Zhang, H., Milon, E., and 15 others. RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression of Notch signaling via histone demethylase KDM1A/LSD1. EMBO J. 36: 3232-3249, 2017. 3. Zhang, C., Leng, F., Saxena, L., Hoang, N., Yu, J., Alejo, S., Lee, L., Qi, D., Lu, F., Sun, H., Zhang, H. Proteolysis of methylated SOX2 protein is regulated by L3MBTL3 and CRL4-DCAF5 ubiquitin ligase. J. Biol. Chem. 294: 476-489, 2019.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.