Recombinant Human HMGB1
Recombinant Human HMGB1
Product Specifications
Product Name Alternative
High mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1
UniProt
P09429
Expression Region
1-215
Reactivity
Human
Tag
C-terminal His Tag
Source
HEK293 cells
Purity
Greater than 90% as determined by SDS-PAGE
Molecular Weight
26.2 kDa
Storage Conditions
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Notes
For research use only.
Applications Notes
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Tested Applications
ELISA, WB
Preservative
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4
Available Sizes
Frequently Asked Questions
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