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Recombinant Human HMGB1

Recombinant Human HMGB1

Product Specifications

Product Name Alternative

High mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

UniProt

P09429

Expression Region

1-215

Reactivity

Human

Tag

C-terminal His Tag

Source

HEK293 cells

Purity

Greater than 90% as determined by SDS-PAGE

Molecular Weight

26.2 kDa

Storage Conditions

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.

Notes

For research use only.

Applications Notes

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Tested Applications

ELISA, WB

Preservative

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4

Available Sizes

Frequently Asked Questions

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