Recombinant Human Leptin
Recombinant Human Leptin
Product Specifications
Product Name Alternative
LEP, leptin, OB, Obese protein, Obesity factor, OBS
UniProt
P41159
Expression Region
22-167
Reactivity
Human
Tag
C-terminal His Tag
Source
E.Coli
Field of Research
Metabolism Research
Purity
Greater than 90% as determined by SDS-PAGE
Molecular Weight
17.8 kDa
Storage Conditions
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Notes
For research use only.
Applications Notes
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Tested Applications
ELISA, WB
Preservative
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4
Available Sizes
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