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Anti-CAMKIV/Camk4 Antibody Picoband®, Dylight594 Conjugate

Boster Bio Anti-CAMKIV/Camk4 Antibody Picoband® catalog # A01905-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Calcium/calmodulin-dependent protein kinase type IV is an enzyme that in humans is encoded by the CAMK4 gene. The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

Synonyms

C-C chemokine receptor type 1;C-C CKR-1;CC-CKR-1;CCR-1;CCR1;Macrophage inflammatory protein 1-alpha receptor;MIP-1alpha-R;RANTES-R;CD191;Ccr1;Cmkbr1;

Gene Name

C-C chemokine receptor type 1

Gene ID

12326

UniProt

P08414

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Camk4 recombinant protein (Position: A19-Y469).

Clonality

Polyclonal

Tissue Specificity

Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for a C-C type chemokine. Binds to MIP-1-alpha, RANTES, and less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

References & Citations

1. Kang, H., Sun, L. D., Atkins, C. M., Soderling, T. R., Wilson, M. A., Tonegawa, S. An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory. Cell 106: 771-783, 2001. 2. Koga, T., Ichinose, K., Mizui, M., Crispin, J. C., Tsokos, G. C. Calcium/calmodulin-dependent protein kinase IV suppresses IL-2 production and regulatory T cell activity in lupus. J. Immun. 189: 3490-3496, 2012. 3. Raman, V., Blaeser, F., Ho, N., Engle, D. L., Williams, C. B., Chatila, T. A. Requirement for Ca(2+)/calmodulin-dependent kinase type IV/Gr in setting the thymocyte selection threshold. J. Immun. 167: 6270-6278, 2001.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

40895 MW

Observed Molecular Weight

58 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse<br> Immunofluorescence, 5μg/ml, Rat<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Mouse<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Cell membrane; Multi-pass membrane protein.

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