Anti-CGAS Antibody Picoband®, Dylight594 Conjugate

Boster Bio Anti-CGAS Antibody Picoband® catalog # A31676-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

cGAS (cyclic GMP-AMP synthase), also known as MB21D1 (Mab-21 domain containing 1), h-cGAS or C6orf150, is a 522 amino acid cytoplasmic nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAS is suggested to have antiviral activity by acting as a key cytosolic DNA sensor. cGAS binds to cytosolic DNA, which leads to cGAMP synthesis and activation of TMEM173, thereby trigger type-I interferon production. Expressed in monocytic cell line THP1, cGAS exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 6q13.

Synonyms

Lymphocyte antigen 6A-2/6E-1;Ly-6A.2/Ly-6E.1;Stem cell antigen 1;SCA-1;T-cell-activating protein;TAP;Ly6a;Ly6;

Gene Name

Lymphocyte antigen 6A-2/6E-1

Gene ID

115004

UniProt

Q8N884

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CGAS recombinant protein (Position: L208-F522).

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

T-cell activation.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

References & Citations

1. Ablasser, A., Hemmerling, I., Schmid-Burgk, J. L., Behrendt, R., Roers, A., Hornung, V. TREX1 deficiency triggers cell-autonomous immunity in a cGAS-dependent manner. J. Immun. 192: 5993-5997, 2014. 2. Ablasser, A., Schmid-Burgk, J. L., Hemmerling, I., Horvath, G. L., Schmidt, T., Latz, E., Hornung, V. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature 503: 530-534, 2013. 3. Andreeva, L., Hiller, B., Kostrewa, D., Lassig, C., de Oliveira Mann, C. C., Drexler, D. J., Maiser, A., Gaidt, M., Leonhardt, H., Hornung, V., Hopfner, K.-P. cGAS senses long and HMGB/TFAM-bound U-turn DNA by forming protein-DNA ladders Nature 549: 394-398, 2017.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

14377 MW

Observed Molecular Weight

62 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -