Anti-MEKK2/MAP3K2 Antibody Picoband®, Dylight594 Conjugate

Boster Bio Anti-MEKK2/MAP3K2 Antibody Picoband® catalog # A02354-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

MAP3K2, mitogen-activated protein kinase kinase kinase 2, also known as MEKK2, belongs to the mitogen-activated protein kinase (MAPK) kinase kinase gene family, which is involved in regulating multiple MAPK signaling pathways. Endogenous MAP3K2 localized mainly to the cytosol of unstimulated HeLa cells and rat fibroblasts. The MAP3K2 gene is mapped to chromosome 2 by the International Radiation Hybrid Mapping Consortium. MAP3K2 is a major upstream activator of the JNK, MAPK cascade. MAP3K2 was identifies as 1 of 6 kinases that regulate different steps of the caveolar cycle. Silencing of MAP3K2 strongly reduced kiss-and-run dynamics, leading to an accumulation of caveolar structures at the cell surface without affecting caveolae coat assembly or clustering.

Synonyms

Calcitonin; Calca; Calc

Gene Name

calcitonin/calcitonin-related polypeptide, alpha

Gene ID

10746

UniProt

Q9Y2U5

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MEKK2/MAP3K2 recombinant protein (Position: M1-Q364).

Clonality

Polyclonal

Applications

Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Yao Z, et al. Calcium regulation of EGF-induced ERK5 activation: role of Lad1-MEKK2 interaction. PLoS One, 2010 Sep 7. 2. Cazares LH, et al. Maging mass spectrometry of a specific fragment of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2 discriminates cancer from uninvolved prostate tissue. Clin Cancer Res, 2009 Sep 1.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

78 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, Human