Anti-FGF21 Antibody , Dylight594 Conjugate

Boster Bio Anti-FGF21 Antibody Picoband® catalog # A00802-1. Tested in ELISA applications. This antibody reacts with Human.

Product Specifications

Background

FGF21 (Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor (FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.

Synonyms

Fibroblast growth factor 21;FGF-21;FGF21;UNQ3115/PRO10196;

Gene Name

Fibroblast growth factor 21

Gene ID

26291

UniProt

Q9NSA1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human FGF21 recombinant protein (Position: H29-S209).

Clonality

Polyclonal

Tissue Specificity

Detected in blood plasma (at protein level). .

Applications

ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. .

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Galman, C., Lundasen, T., Kharitonenkov, A., Bina, H. A., Eriksson, M., Hafstrom, I., Dahlin, M., Amark, P., Angelin, B., Rudling, M. The circulating metabolic regulator FGF21 is induced by prolonged fasting and PPAR-alpha activation in man. Cell Metab. 8: 169-174, 2008. 2. Hondares, E., Rosell, M., Gonzalez, F. J., Giralt, M., Igelsias, R., Villarroya, F. Hepatic FGF21 expression is induced at birth via PPAR-alpha in response to milk intake and contributes to thermogenic activation of neonatal brown fat. Cell Metab. 11: 206-212, 2010. 3. Nishimura, T., Nakatake, Y., Konishi, M., Itoh, N. Identification of a novel FGF, FGF-21, preferentially expressed in the liver. Biochim. Biophys. Acta 1492: 203-206, 2000.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.