Bisindolylmaleimide X (hydrochloride)

Bisindolylmaleimide X hydrochloride (Ro 31-8425 hydrochloride, BIM-X hydrochloride) is a cell-penetrating PKC inhibitor. Bisindolylmaleimide X hydrochloride is a potent cyclin-dependent kinase 2 (CDK2) antagonist with an IC50 of 200 nM. Bisindolylmaleimide X hydrochloride inhibits the proliferation of CD4 T cells in vitro. Bisindolylmaleimide X hydrochloride inhibits eNOS-Ser1177 phosphorylation in human embryonic vein endothelial cells. Bisindolylmaleimide X hydrochloride can be used for research on the immune system and cardiovascular diseases[1][2][3].

Product Specifications

CAS Number

[145317-11-9]

Product Name Alternative

BIM-X (hydrochloride) ; Ro31-8425 (hydrochloride)

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

CDK; PKC

Type

Reference compound

Related Pathways

Cell Cycle/DNA Damage; Epigenetics; TGF-beta/Smad

Applications

Neuroscience-Neuromodulation

Field of Research

Cancer; Cardiovascular Disease; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/bisindolylmaleimide-x-hydrochloride.html

Purity

98.90

Solubility

DMSO : 20.83 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C(C(C1=C(CC(CN)CC2)N2C3=C1C=CC=C3)=C4C5=CN(C)C6=C5C=CC=C6)NC4=O.[H]Cl

Molecular Formula

C26H25ClN4O2

Molecular Weight

460.96

Precautions

H302, H315, H319, H335

References & Citations

[1]Levy O, et al. A cell-based drug delivery platform for treating central nervous system inflammation. J Mol Med (Berl) . 2021 May;99 (5) :663-671.|[2]Song W, et al. Icariside II induces rapid phosphorylation of endothelial nitric oxide synthase via multiple signaling pathways. PeerJ. 2022 Oct 25;10:e14192. |[3]Brehmer D, et al. Proteome-wide identification of cellular targets affected by bisindolylmaleimide-type protein kinase C inhibitors. Mol Cell Proteomics. 2004 May;3 (5) :490-500.