Human IgM (myeloma)

Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (μ chain specific) . No reaction was observed against anti-Human IgG F (c) . Some light chain cross-reactivity will occur with anti-Human IgG.

Store vial at 4° C prior to opening. Human IgM (myeloma) whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Human IgM whole molecule has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting