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Sheep IgG

Sheep IgG

Product Specifications

Product Name Alternative

Sheep Immunoglobulin Gamma, Immunoglobulin G

Conjugation

Unconjugated

Source

Sheep

Origin

Sheep

Field of Research

Protein Biochemistry

Concentration

10.0 mg/mL

Dilution

ELISA: User Optimized, IHC: User Optimized, WB: User Optimized

Purity

Sheep IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Sheep IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG and anti-Sheep Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Sheep IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Sheep IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 2.5 mL

Tested Applications

SDS-PAGE

Preservative

Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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