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Anti-Bik Antibody Picoband® (Dylight594 Conjugate)

Boster Bio Anti-Bik Antibody Picoband® catalog # PB9755. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Synonyms

Bcl-2-interacting killer;Apoptosis inducer NBK;BIP1;BP4;BIK;NBK;

Gene Name

Bcl-2-interacting killer

UniProt

Q13323

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with mouse Bik.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .

Applications

Flow Cytometry,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X (L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PB9755

Product MSDS

https://www.bosterbio.com/msds?sku=PB9755

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat <br>Western blot, 0.1-0.5μg/ml, Human, Rat<br>Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human<br> Immunocytochemistry, 0.5-1μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human

Subcellular Location

Endomembrane system; Single-pass membrane protein. Mitochondrion membrane ; Single-pass membrane protein . Around the nuclear envelope, and in cytoplasmic membranes.

Protein Name

Bcl-2-interacting killer

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