Anti-MISP Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-MISP Antibody Picoband® catalog # A08017-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The protein encoded by this gene is an actin-bundling protein involved in determining cell morphology and mitotic progression. The encoded protein is required for the proper positioning of the mitotic spindle. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Synonyms

BMP and activin membrane-bound inhibitor homolog; Non-metastatic gene A protein; Putative transmembrane protein; NMA; BAMBI; NMA

Gene Name

BMP and activin membrane bound inhibitor

Gene ID

126353

UniProt

Q8IVT2

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MISP recombinant protein (Position: M1-R632).

Clonality

Polyclonal

Tissue Specificity

Expressed in adult liver.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Negatively regulates TGF-beta signaling.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 6/26/2013. 2. Maier, B., Kirsch, M., Anderhub, S., Zentgraf, H., Kramer, A. The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells. Cell Cycle 12: 1457-1471, 2013. 3. Zhu, M., Settele, F., Kotak, S., Sanchez-Pulido, L., Ehret, L., Ponting, C. P., Gonczy, P., Hoffmann, I. MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression. J. Cell Biol. 200: 773-787, 2013.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

17136 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -